Detection of Rice Stripe Virus using RT-PCR
نویسندگان
چکیده
منابع مشابه
Detection of Avian Encephalomyelitis Virus in Broiler Chickens in Iran Using RT-PCR and Histopathological Methods
Avian encephalomyelitis is caused by a Tremovirus and primarily affects chickens. The virus can infect young chicks and cause nervous symptoms. Vaccines are used to control the disease in breeders. Recently, the occurrence of the disease is causing concern in the poultry industry. In the present study, we report a case of avian encephalomyelitis in one broiler farm in Kashan (center of Iran). T...
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Hepatitis Delta virus (HDV) is a degenerate RNA virus or virusoid and a satellite of Hepatitis B virus (HBV). Three distinct genotypes are described for HDV; genotype I is distributed worldwide but other genotypes appear to be more restricted geographically. In the present study, an RT-nested PCR method was set up to detect delta infection from serum samples. Moreover, the target amplified sequ...
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Miranda Gilda Jonson, Sen Lian, Hong-Soo Choi, Gwan-Seok Lee, Chang-Suk Kim and Kook-Hyung Kim* 1Department of Biology, School of Science and Engineering, Ateneo de Manila University, Katipunan Avenue, Loyola Heights Quezon City 1108, Philippines 2Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National Universi...
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Maize stripe virus (MStpV) and rice stripe virus (RSV) were compared serologically, chemically and physically. Cross-reactions in agar gel double-diffusion and microprecipitin tests, and neutralization of MStpV infectivity by antiserum to either virus showed that MStpV and RSV are serologically related. Both viruses sedimented slowly and in a heterodisperse manner in rate-zonal sucrose gradient...
متن کاملDetection and quantitation of bovine respiratory syncytial virus using real-time quantitative RT-PCR and quantitative competitive RT-PCR assays.
A single tube, fluorogenic probe-based, real-time quantitative reverse transcription-polymerase chain reaction (Q-RT-PCR) assay was developed for detection and quantitation of bovine respiratory syncytial virus (BRSV) using BioRad's iCycler iQ. Real-time Q-RT-PCR was compared with quantitative competitive RT-PCR (QC-RT-PCR) and viral titers. Viral mRNA levels were measured in BRSV-infected bovi...
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ژورنال
عنوان ژورنال: Research in Plant Disease
سال: 2004
ISSN: 1598-2262
DOI: 10.5423/rpd.2004.10.1.030